Platelet derived growth factor down-regulated sarcoplasmic reticulum Ca2+atpase to promote the phenotype modulation of human airway smooth muscle cells
ZHONG Xiao-hong
CHENG Yuan-xiong
YAO Zhi-hui
YUAN Ya-lu
CAO Jing
LAI Wen-yan
CAI Kai-can
Abstract：Objective To investigate of the effect and mechanisman of SERCA2 on the phenotype modulation of HASMCs. Methods HASMCs were starved for 5 days and divided into different groups ,then we observed morphology change of the cells from the microscope and detected a-actin、SERCA2 and p-ERK by Western Blot,cells proliferation was observed by CCK-8 method. Results Compared with the control group,PDGF could reduce a-actin of HASMCs and increased the cells proliferation ability ,TSG could significantly inhibit the effect (P<0.01), PDGF could also significantly inhibit SERCA2 protein and increased the expression p-ERK (P<0.01), while U0126 significantly inhibited the effect (P < 0.01). Conclusion PDGF may induce HASMCs phenotype modulation through the regulation of SERCA2 and p-ERK.
Keywords：Human airway smooth muscle cellsCell phenotypeSarcoplasmic reticulum Ca2+atpaseERK pathway
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 2133-2137 )