Effect of 5-Aza-CdR with MS275 on growth and expression of DNMT1 protein in cell lines of non-small cell lung cancer
LIU Bang-qing
LUAN Jia-qiang
SUN Xiao-lin
BAI Jian
SONG Jian-fei
DU Zhen-zong
Abstract：Objective To explore the characteristics and biological functions of epigenetic treatment to DNMT1 expression in NSCLC cell lines. Methods Lung cancer cells were divided into 4 groups , and drugs with different concentrations. The effect of epigenetic treatment on NSCLC cell line was detected by CCK-8 and MTT. The effect of epigenetic treatment on the expression of DNMT1 in NSCLC cell line was detected by Western blot. Results CCK-8 and MTT assay showed that treatment with 5-Aza-CdR and MS-275 inhibited the proliferation of NSCLC cells. Western blot showed that treatment with 5-Aza-CdR and MS-275 inhibited the DNMT1 expression in NSCLC cells. Conclusion The expression of DNMT1 gene in the NSCLC cell lines may be suppressed effectively by epigenetic treatment , and the gene may inhibit the proliferation and growth of NSCLC cell lines.
Keywords：Non-small cell lung cancerEpigenetic treatmentDNA methyltransferase 1DNA methylation
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 2105-2108 )