Effects of IFN-γ on airway inflammation and protein MUC5ac mRNA expression in mice with asthma
Abstract：Objective To investigate the effects of IFN-γon airway inflammation and protein MUC5ac mRNA expression in mice with asthma. Methods Forty c57bL/6 mice were randomly divided into control group, model group, IFN-γ 50,000IU group and IFN-γ 100,000IU group, 10 for each group. The change rate of airway resistance was detected after acetylcholine chloride provocation. The count of inflammatory cells in BALF was analyzed. Levels of IL-13 in BALF were measured by ELISA. The lung tissue sections were stained with Ab-PAS for identification of goblet cells proliferation. Expressions of MUC5ac mRNA in the lung tissues were determined by real time-PCR. Results As compared with the control group , the change rate of airway resistance , count of inflammatory cells , levels of IL-13 and MUC5ac mRNA in lung were significantly increased in the model group (P < 0.05). IFN-γ reduced airway resistance, inflammatory cells, levels of IL-13 and MUC5ac mRNA in the lungs (P < 0.05). Conclusions IFN-γ can regulate the balance of Th1/Th2, inhibit airway goblet cell proliferation , and protein MUC5ac mRNA expression in mice withasthma.
Keywords：AsthmaIFN-γGoblet cellsIL-13MUC5ac
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 3129-3132 )