Neuroprotection of selective antegrade cerebral perfusion on pig model exposed to deep hypothermia circulatory arrest
LIU Zi-you
LIANG Meng-ya
CHEN Guang-xian
TANG Zhi-xian
RONG Jian
YAO Jian-ping
YANG Xiao
WU Zhong-kai
Abstract：Objective To explore the expression of TLR4/NF-κB pathway in cerebral injury resulting from DHCA ( deep hypothermia circulatory arrest ) as well as the effect of SACP ( selective antegrade cerebral perfusion). Methods Twelve pigs were randomly assigned to DHCA group (n = 6) or SACP group (n = 6) at 18 ℃ for 80 min. IL-6 was assayed by ELISA. Apoptosis and NF-κB proteins were detected by fluorescence TUNEL and Western blot, respectively. The level of TLR4 was determined through qRT-PCR and Western blot. Results Serum IL-6 level of SACP group was significantly lower at the end of circulation arrest and experiment and apoptotic index and NF-κB protein were apparently lower in SACP group (P < 0.05). The level of TLR4 protein and mRNA from SACP group decreased significantly (P < 0.05). Conclusions TLR4/NF-κB pathway plays a critical role in pathogenesis of DHCA cerebral injury and attenuating TLR4/NF-κB cytokines probably contributes to neuroprotection of SACP. TLR4/NF-κB pathway may be a novel target for DHCA.
Keywords：Circulatory arrestdeep hypothermia inducedSelective antegrade cerebral perfusionTLR4NF-κBIL-6Brain injuries
Publication Date：2015-03-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 693-696 )