Inhibition effect of indirubin on early brain injury after spontaneous subarachnoid hemorrhage

doi:10.3969/j.issn.1006-5725.2014.24.013

Abstract：Objective To investigate the inhibition effect of indirubin on early brain injury after spontaneous subarachnoid hemorrhage. Methods Ninety male adult C57BL/6J mice were randomly divided into 3 groups:sham group, SAH+vehicle group and SAH+Indirubin group. Subarachnoid hemorrhage model was established by endovascular puncture method, and the blood flow of bleeding cerebral hemisphere was measured by laser Doppler flowmeter. The wet-dry weighing method was performed to measure the brain water content.The neurological appraisal was evaluated by neurological score. The apoptosis of neuron in the cortex and hippocampus was examined by TUNEL staining. Results SAH total mortality was 23.3%. Mortality showed statistical difference between SAH+vehicle group (24.1%) and SAH+Indirubingroups (21.8%).The neurological scores of the SAH+vehicle group were 11.13 ± 2.45、13.81 ± 2.46 at 24, 72 h, which was higher than SAH+Indirubin group(12.55 ± 2.11、16.32 ± 1.09). There were significant difference between groups at the same time point(P<0.05). At the bleeding cerebral hemisphere, brain water content of SAH+ Indirubin group was (77.57 ± 0.35)%, which was better than SAH+vehicle group (78.12 ± 0.47)%(P<0.05). The TUNEL positive cells rate of Cortex and hippocampus in SAH+Indirubin group are lower than SAH+vehicle group (P<0.05). Conclusion Indirubin can relieve cerebral edema,reduce apoptosis of the cerebral cortex and hippocampal neurons, and improve early neurological function of SAH mice.