Study on the protecting function of Berberine on the invasion of Streptococcus pneumoniae to A549 cells in vitro
WANG Rong
XU Bang-lao
Abstract：Objective To investigate whether Berberine can inhibit the invasion of Streptococcus pueum oniae ( S.pn) to A549 cells in vitro. Methods A549 cells were pretreated with the different concentrations of Berberine and incubated together with S.pn. A549 cells were lysed with 0.025%Triton X-100.Diluted lysate was streaked on TSAB plate and incubated overnight. The colonies on the plate were counted. Results The counts on the plates were 52 ± 21,29 ± 11,13 ± 10,0,0 per well when the concentration of Berberine was 0,25,50,100,200μg/mL, respectively. No invasion was observed when the concentration of Berberine was 100 μg/mL. Conclusion Berberine can inhibit the invasion of S.pn to A549 cells in dose-dependent maner in vitro.
Keywords：Streptococcus pneumoniaTypeⅡpneumocyteInvasion
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：2( 2538-2539 )