Effects of hypertonic saline on TNF-α、IL-1β、S-100β following focal cerebral ischemia-reperfusion injury in rats
LAI Yong-dong
JIN Wen-xiang
HE Rong-zhi
Abstract：Objective To investigate the effect of hypertonic saline on TNF-а, IL-1β, S-100β level following focal cerebral ischemia-reperfusion injury in rats and explore the mechanisms involved. Methods Ninety rats were randomized equally into 4 groups,namely the pseudo-operated group (A group),untreated IR injury group (B group),C group (4.2% hypertonic saline) and D group (7.5% hypertonic saline). Cerebral ischemia was induced by middle cerebral artery occlusion for 2 h followed by administration of the corresponding treatments in group C and D. After 22 h of reperfusion , TNF-а, IL-1β, S-100βexpression in the ischemic brain tissue were measured by enzyme-linked immunosorbent assay (ELISA). Results After hypcrtonic saline treatment, the two saline-treated groups showed significant reduction in TNF-а、IL-1β、S-100β levels , but were still higher than pseudo-operated group(A group). Compared with B group, significant difference can be seen among C and D group. Conclusion Hypcrtonic saline can reduce cerebral TNF-а、IL-1β、S-100βlevel of isehemia-reperfusion injury.
Keywords：Isehemia-reperfusionHypertonic salineTNF-αIL-1βS-100β
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 2532-2534 )