The influence of inactive frmd4a gene on the biological behavior of human tongue cancer cal-27 cell
ZHENG Xiang-huai
ZHAO Jian-Jiang
JIA Bo
PAN Jie
CHEN Jun
QIU Xiao-ling
HAN Jiu-Song
CHU Hong-Xing
Abstract：Objective To observe the influence of inactive FRMD4A gene′s expression on the biological behavior of tongue cancer CAL-27cell. Methods FRMD4A-siRNA was transfered into CAL-27 cell by lipidosome, to the expression of FRMD4A-siRNA in CAL-27 cell after transfection was detect by qRT-PCR cell proliferation , was checked by CCK-8,the influence of inactive FRMD4A gene on cell cycle distribution of CAL-27 cell was assayed by flow cytometry. Results Compared with the control group, FRMD4A mRNA expression significantly reduced in FRMD4A-siRNA interfering group (94%) and the cell proliferation index decreased(P<0.05). The cell cycle arrested in G1 period (P<0.05). Conclusion FRMD4A-siRNA could effectively inhibit FRMD4A mRNA expression in tongue cancer CAL-27cell, impact the distribution of cell cycle, and reduce cell proliferation.
Keywords：Tongue neoplasmsGene silencingFRMD4ACell proliferationCell cycleWound healing
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 1541-1544 )