The Effect of Candesartan on human breast cancer cell line MCF-7
Abstract：Objective To investigate the proliferation inhibition of angiotensin Ⅱ 1 receptor (AT1R) antagonist candesartan on human breast cancer cell line MCF-7. Methods MCF-7 cells were tested in vitro for cytotoxicity of candesartan with MTT method. The effect of candesartan on cell cycle and apoptosis of MCF-7 cells were evaluated by flow cytometry. Western Blot was applied to test the expression level of wild-type p53 and Survivin protein. Results After candesartan treatment, a dose- and time-dependent decreased proliferation in MCF-7 cells was observed. Induction of G1 cell cycle arrest and increased apoptosis in MCF-7 cells were also observed.Western blot assay showed that candesartan up-regulated wild-type P53 expression and down-regulated the Survivin expression in MCF-7 cells. Conclusions This study suggests that candesartan could inhibit the proliferation , induce G1 cell cycle arrest and apoptosis of MCF-7cellsby regulating the protein expression level of gene p53 and Survivin.
Keywords：Breast carcinomaCandesartanCell cycle arrestApoptosisp53Survivin
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 511-514 )