Effects of Rab25 gene on the growth of implanted tumor of breast cancer in nude mice
ZHANG Xiao-bin
LU Yun-fei
SHEN Gui-xin
WU Fei
Abstract：Objective To establish nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene , and to observe the effects of Rab25 gene on the growth of implanted tumor. Methods Nude mice models with implanted tumor of breast cancer cells with different expressing levels of Rab25 gene were constructed through up-regulating or silencing the expression of Rab25 gene in breast cancer cells. The size of implanted tumor was measured, the growth curve of tumor was portrayed, and the expression of Rab25 gene in tumor tissues was detected. Results The tumorigenic effect on nude mice was the strongest and the highest in Rab25 up-regulating group , as compared to those in Rab25 interference group , original cells group, and Rab25 empty plasmid group. The expression of Rab25 mRNA in interference group was significantly reduced. Conclusions Rab25 is a cancer-promoting gene , its high expression could directly enhance the proliferation of breast cancer cells. RNA interference technology had the potential to provide new ideas for tumor targeting and gene therapy for breast cancer.
Keywords：Breast neoplasmsRab25Nude miceImplanted tumor
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 361-364 )