Inhibitory effect of human umbilical cord mesenchymal stem cells on proliferation and migration of esophageal cancer EC9706 cells and the transplanted tumor in nude mice
YAO Wen-jian
BAI Yu
ZHAO Bao-sheng
LIU Shang-guo
QI Bo
Abstract：Objective To investigate the inhibitory effect of human umbilical cord mesenchymal stem cells(hUCMSCs) on proliferation and migration of esophageal cancer EC9706 cells and the transplanted tumor in nudemice. Methods hUCMSCs were isolated and cultured in vitro. The phenotype of hUCMSCs was identified.hUCMSCs were indirectly co-cultured with EC9706 and the effect of co-culture on EC9706 proliferation andmigration was evaluated. Animal models of transplanted tumor were established in nude mice. hUCMSCs wereinfused via the tail vein and the effect of hUCMSCs on the tumor growth and metastasis was investigated. ResultsAt 9 to 14 days after cell adherence, the fibroblast-like cells were observed. Flow cytometry analysis revealed thatCD29 and CD44 were highly expressed on the plasma membrane of the passages 3 cells, with negative expression ofCD34, CD45 and HLA-DR. Results of MTT assay and cell scratch test showed that compared with the control group,the proliferation and migration of EC9706 in the experimental group were significantly inhibited, respectively. Theaverage tumor weight in the control group and in the experimental was (1.83 ± 0.33)g and (1.02 ± 0.15)g,respectively. The average number of metastasis nodules was 5.37 ± 1.12 and 1.29 ± 1.05 respectively, withsignificant difference. Conclusion hUCMSCs can inhibit the proliferation and migration of EC9706 cells andinhibit the growth and metastasis of transplanted tumor in nude mice.
Keywords：Esophageal neoplasmsHuman umbilical cord mesenchymal stem cellsMigrationNude mice
Publication Date：2014-01-31
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 354-356 )