Reversion of multidrug resistance of non-small cell lung cancer by targeted blocking Snail
ZHANG Li-na
LIU Fei
SUN Lei
TIAN Hui-ling
CHEN Wei-yi
LI Hong-li
ZHANG Bao-gang
YIN Chong-gao
GUO Wen-jun
Abstract：Objective To investigate the correlation between the epithelial-mesenchymal transition (EMT) and multidrug resistance (MDR) gene in patients with non-small cell lung cancer (NSCLC), and to provide experimental evidence for clinical treatment for NSCLC. Methods Immunohistochemistry SP method was used to detect the expressions of Snail, P-glycoprotein (P-gp) and lung resistance protein (LRP) in 52 patients with NSCLC, and their correlations were analyzed. Stable knockout of Snail in A549 cells by RNA interference was administered, and then Western blot was used to analyze the expressions of Snail, P-gp and LRP protein in A549 cells. Results The expression rates of Snail, P-gp and LRP in NSCLC tissues were 73.1%, 53.8% and 51.9%, respectively. The expression of Snail was significantly related to those of P-gp and LRP. Successful knockout of Snail gene in A549 cells by RNAi technology was observed , and then the expressions of P-gp and LRP were significantly down-regulated. Conclusion In NSCLC, the expression of Snail protein is significantly associated with MDR related gene. Blocking the expression of Snail can effectively reverse the MDR in A549 cells.
Keywords：Carcinomanon-small-cell lungTranscription factorSnailEpithelial-mesenchymal transitionP-gpLRP
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 345-348 )