Effect of 1,25-dihydroxyvitamin D3 on the proliferation and apoptosis of human glomerular mesangial cells

CHEN Jian-ping; ZHANG Chun-jiang; ZHAO Dan; MA Li; YIN Xuan; YANG Xiao-ping

doi:10.3969/j.issn.1006-5725.2014.03.003

Abstract：Objective To investigate the effects of 1,25-dihydroxyvitamin D3 on the proliferation and apoptosis of cultured human glomerular mesangial cells. Methods The cultured human glomerular mesangial cells were divided into four groups: the normal control group, the EGF (10 ng/mL) group, the 1,25-dihydroxyvitamin D3 (10-8 mol/L) group and the EGF in combination with 1,25-dihydroxyvitamin D3 group. Afterr 48-hours treatment, cell proliferation was measured by cell counting kit-8 (cck-8) assay, cell cycle was measured by flow cytometry, and cell apoptosis was measured by the TdT-mediated dUTP nick-end labeling (TUNEL) assay. Results Compared with the normal control, EGF could significantly promote the proliferation of human glomerular mesangial cells (P<0.01). The percentage of cells with G1 phase significantly decreased and the percentage of cells with S phase significantly increased (P < 0.01). The apoptosis index of the human glomerular mesangial cells significantly decreased in the EGF group (P < 0.01). 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation of human glomerular mesangial cells (P<0.01). 1,25-dihydroxyvitamin D3 could significantly increase the percentage of G1 phase cells and could decrease the percentage of S phase cells (P<0.01), and could significantly increase the apoptosis index of human glomerular mesangial cells (P<0.01). Compared with EGF, EGF in combination with 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation of human glomerular mesangial cells (P <0.01). The percentage of G1 phase cells significantly increased and the percentage of S phase cellssignificantly decreased (P < 0.01), and the cell apoptosis index significantly increased in the EGF in combination with 1,25-dihydroxyvitamin D3 group (P < 0.05). Conclusions (1) 1,25-dihydroxyvitamin D3 could significantly inhibit the proliferation and promote the apoptosis of human glomerular mesangial cells. (2) 1,25-dihydroxyvitamin D3 could also inhibit the proliferation of EGF-induced human glomerular mesangial cells.