Effects of Non-crosslinked Sodium Hyaluronate Solution Combined with Nicotinamide and Amino Acids on Melanin Inhibition and Collagen Regeneration

LIU Mouzhi; JIN Yu; XU Lin; JIANG Yiyuan; XU Songquan; XU Nianqin

doi:10.11786/sypfbxzz.1674-1293.20250504

Abstract：Objective To investigate the melanin-inhibiting and collagen-promoting effects of non-crosslinked sodium hyaluronate(SH)solution combined with nicotinamide and amino acids.Methods First,the concentration of nicotinamide in the SH complex solution was determined using a mouse melanoma cell(B16-F10 cell)melanin inhibition model.Then,the effects of the non-crosslinked SH solution combined with nicotinamide and amino acids on tyrosinase activity and melanin production were evaluated using a zebrafish model.The effects of the SH complex solution on the viability of Haman skin fibroblasts HSF and collagen content were detected,and the moisturizing effect of the SH complex solution was verified through clinical trials.Results The SH solution with nicotinamide significantly inhibited melanin production in B16-F10 cells.When the addition amount was above 1.25 mg/mL,the inhibition rate of melanin production in B16-F10 cells exceeded 20％,but the effect on cellular tyrosinase activity was not significant.The SH complex solution significantly inhibited both tyrosinase activity and melanin production in zebrafish,with the 8％SH complex solution group showing inhibition rates of 21.3％and 50.6％,respectively.Meanwhile,the SH complex solution significantly promoted HSF proliferation and collagen regeneration.Compared with the normal group,the 10％SH complex solution group increased cell growth by 35.7％and 28.0％after 24 h and 48 h of cell treatment,respectively,and the SH solution increased cellular collagen synthesis by 98.80％after 48 h of cell treatment.Clinical trial results showed that the SH complex solution had a significant moisturizing effect.One week after administration of the SH complex solution,the water content in the middle of the forehead,the middle of the right face,the middle of the left face,and the middle of the jaw of the subjects was significantly higher than before injection(P＜0.05),with increase rates of 32.48％,20.11％,28.01％,and 31.05％,respectively.Moreover,the skin dryness improvement effect score one week after injection signficantly lower than before injection(P＜0.05).Conclusion The non-crosslinked SH complex solution has obvious effects of inhibiting melanin production and promoting collagen regeneration,and has a good moisturizing effect.