Preliminary Study of Psoriasis-related Cuproptosis Genes Based on Bioinformatics Methods
MA Xiao
XU Haoxiang
HE Yanyan
JI Juan
SUN Weiguo
KONG Yinghui
Abstract：Objective To screen psoriasis-related cuproptosis genes,and to explore possible regulatory mechanisms using bioinformatics methods.Methods A psoriasis dataset GSE 30999 was downloaded from the Gene Expression Omnibus(GEO)database to screen differentially expressed genes(DEGs)between lesional and non-lesional skin tissues.Gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analysis were carried out to identify biological functions and enriched pathways of the DEGs,respectively.Immune infiltration analysis was used to obtain the distribution of immune cells in psoriasis tissues.DEGs were intersected with previously reported cuproptosis genes,thus screening out the cuproptosis-related differentially expressed genes(CR-DEGs).Molecular docking with acitretin was utilized to verify its binding activity with CR-DEGs.The single-cell dataset GSE 230842 was used for cell clustering analysis to clarify the distribution of CR-DEGs in key cell clusters.Results A total of 2,061 DEGs were obtained.GO analysis showed that DEGs were mainly enriched in iron ion binding and fatty acid metabolic process,while KEGG pathway enrichment analysis revealed that the DEGs were significantly enriched in cytokine-cytokine receptor interactions,and PPAR signalling pathways.The results of immune infiltration indicated that the immune cells,such as CD4+T cells and γδT cells were significantly elevated in psoriasis lesions(P＜0.05).There were 19 cuproptosis genes,and two CR-DEGs,namely DLAT and SLC31A1,were screened by taking an intersection.Single-cell analysis showed that they were abundantly expressed in keratinocytes.The results of molecular docking showed that acitretin well bound with these two genes,especially with SLC31A1.Conclusion DLAT and SLC31A1,as psoriasis-associated cuproptosis genes,may affect the occurrence and development of psoriasis by regulating the proliferation and differentiation of keratinocyte and promoting neovascularization.Acitretin binds strongly to SLC31A1,which may be used for the treatment of psoriasis through the cuproptosis pathway.
Keywords：PsoriasisCuproptosisDifferentially expressed genesBioinformatics
Publication Date：2025-10-10
Online Publishing Date：2025-11-05(First online date of this platform, not the publication date of the document)
Pages：8( 329-336 )