Correlation of heparanase and PI3K/AKT pathway expression in cutaneous squamous cell carcinoma and its clinical significance

LV Ming-jun; QI Dong-wei

doi:10.11786/sypfbxzz.1674-1293.20230601

Abstract：Objective To study the correlation and clinical significance of heparanase(HPA)and PI3K/AKT pathway expression in cutaneous squamous cell carcinoma(CSCC).Methods Eighty-six cases of CSCC and sixty-two cases of normal skin tissues were collected.The positive expression rate of HPA was detected by immunohistochemistry.The mRNA expression level of HPA was detected by fluorescence quantitative PCR,and the protein expression levels of HPA,p-PI3K and p-AKT were detected by Western blot.The difference of HPA expression level between CSCC tissue and normal skin tissue was compared.The correlation between HPA expression and the expression levels of p-PI3K and p-AKT in CSCC tissue was analyzed by Spearman and Pearson test.The prognosis of CSCC patients was followed up and the K-M curve was drawn.The difference in prognosis of CSCC patients with different HPA expression levels was analyzed by log rank test.Results The positive expression rate,mRNA expression level,protein expression level of HPA and the protein expression levels of p-PI3K and p-AKT in CSCC were higher than those in normal skin tissue(P＜0.05).The expression level of HPA in CSCC was positively correlated with the expression levels of p-PI3K and p-AKT(P＜0.05).The positive expression rate,mRNA expression level and protein expression level of HPA in CSCC tissues with moderately differentiation,lymph node metastasis were higher than those in CSCC tissues with highly differentiated,non-lymph node metastasis(P＜0.05).The cumulative overall survival rate of patients with HPA positive expression,mRNA expression level ≥ median and protein expression level ≥ median in CSCC was lower than that of patients with HPA negative expression,mRNA expression level＜median and protein expression level＜median(P＜0.05).Conclusion The increased expression of HPA in CSCC is related to the activation of downstream PI3K/AKT pathway,the decrease of differentiation,lymph node metastasis and poor prognosis.