Effect of 5-ALA-PDT on capability of phagocytosis and the level of ROS of murine macrophage RAW264.7 cells
Abstract：Objective To explore the effect of 5-ALA-PDT on capability of phagocytosis and the level of ROS of murine macrophage RAW264.7 cells. Methods Murine macrophage RAW264.7 cells were induced by inactivated Propionibacterium acnes fluid to establish an inflammatory cell model, then the RAW264.7 cells were treated with different concentrations of 5-ALA combined with infrared radiation with a light dose of 16 J/cm2. The capability of phagocytosis of RAW264.7 cells was detected by using neutral red method. Fluorescence intensity of cellular reactive oxygen species(ROS) was detected by fluorescence microscopy, the level of reactive oxygen species(ROS) was detected by dihydrochloride acetyl acid dichloride. Results The capability of phagocytosis of the model group was higher than that of the blank group. With the increasing concentration of 5-ALA, the capability of phagocytosis decreased in a dose-dependent manner. The result of fluorescence showed that the cells in the 0.12 mmol/L 5-ALA group had strong red fluorescence. With the increasing concentration of 5-ALA, the level of ROS was increased in a dose-dependent manner. Conclusion 5-ALA-PDT can affect the phagocytosis and the level of ROS of murine macrophage RAW264.7 cells, which may affect the efficacy of 5-ALA-PDT.
Keywords：5-aminolevulinic acidPhotodynamic therapyRAW264.7 cellsPhagocytosisReactive oxygen species
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 142-144 )