The experimental study on the toxicity of curcumin combined with thermotherapy on human melanoma A375 cell
HAO Hui-xia
LI Gui-mei
SHA Na
Abstract：Objective To investigate the feasibility of applying curcumin combined with thermotherapy in the treatment of malignant melanoma at the cell level.Methods MTT was used to detect the effects of different treatment factors on cell growth of human malignant melanoma A375 cells,and the apoptosis of A375 cells was detected by flow cytometry.Results The inhibition of 30 μmol/L curcumin combined with thermotherapy was higher than that of simple curcumin group and thermothempy group,the difference was statistically significant (F=5.47,P＜0.01).The inhibition rate ofA375 cells was correlated with the concentration of curcumin (rs=0.95);the apoptosis rate of 30 μmol/L curcumin combined with thermotherapy group was higher than the simple curcumin group and thermotherapy group,the difference was statistically significant (H=15.37,P＜0.01).Conclusion Curcumin combined with thermotherapy has synergistic effects on the cytotoxicity of human malignant melanoma A375 cells,and the cytotoxicity and the dose of curcumin have obvious dose effect relationship.
Keywords：CurcuminThermotherapyCell toxicityMelanomamalignantA375 Cell
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 321-323 )