Effects of GM-CSF on the activities of human monocyte-like THP-1 cells against T. asahii infection

doi:10.11786/sypfbxzz.1674-1293.20170404

Abstract：Objective To investigate the effects of recombinant human Granulocyte-Macrophage Colony-Stimulating Factor (rh GM-CSF) on the phagocytosis and fungicidal activities of human monocyte-like THP-1 cells against Trichosporon asahii(T. asahii) infection. Methods Human monocyte-like THP-1 cells were incubated with various concentrations (100, 200, 400U/ml) of rh GM-CSF or medium alone for 48 h, then co-cultured with T. asahii for 1 h. The phagocytosis activities of THP-1 cells against T. asahii were observed by inverted microscope after stained with Wright-Giemsa stain. The fungicidal activities of THP-1 cells against T. asahii were assessed by counting the number of colony forming units (CFU/ml). Results The phagocytic rate of THP-1 cells to T. asahii (1 h) increased from 20％ to 84％after pre-incubated with rh GM-CSF. The phagocytosis rate of human THP-1 cells to T. asahii increased with the increase of rh GM-CSF concentration in a concentration dependent manner. For 200 U/ml and 400 U/ml rh GM-CSF preincubation groups, each T. asahii colony forming unit was significantly less than the control group. Conclusion GM-CSF can augment the phagocytosis and fungicidal activities of human monocyte-like THP-1 cells against T. asahii. The application of rh GM-CSF may be helpful to overcome the T. asahii infections and represents as a useful therapeutic strategy.