The impairment of immune response against Trichosporon asahii clinical isolate in CARD9 knockout murine bone marrow-derived dendritic cell
Abstract：Objective To investigate the impairment of immune response against Trichosporon asahii(T.asahii) clinical isolate in CARD9 knockout murine bone marrow-derived dendritic cell(BMDC). Methods After in vitro co-culture of BMDCs from wild type mice(WT) and CARD9 knockout mice(CARD9-/-) with heat-killed T.asahii, the phagocytotic ability, costimulatory molecules activation and cytokines production of BMDCs between WT and CARD9-/- were compared. Results Compared with the WT BMDC, the phagocytotic ability of CARD9-/- BMDC was not significantly impaired, but its activation of CD80 and CD86 and the production of IL-6 and TNF-α were significantly lower after co-cultured with T.asahii for 24h. After having been infected by T.asahii, the CARD9-/- mice showed remarkable lower survival rate than the WT mice. Conclusion CARD9-/- BMDC exhibited the defects in costimulatory molecules activation and inflammatory cytokines production but not the phagocytotic ability against T.asahii.
Keywords：Trichosporon asahiiGegeCARD9Dendritic cell
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 73-76 )