Effects of LXRs agonists on the proliferation of SCL-1 cells
Abstract：Objective To detect the effects of LXRs agonists T0901317 on human cutaneous squamous cell carcinoma SCL-1. Methods 24,48 and 72 hours after T0901317(1.0, 10.0, 20.0 μmol/L) with different concentrations acting on SCL-1 cells, CCK-8kit assay was performed to detect cell proliferation. After having been treated with T0901317(1.0, 10.0, 20.0 μmol/L) for 24 hours, SCL-1 cells cycle progression was analyzed by flow cytometry. RT-PCR and Western blot were used to detect the expression of cell cycle related factors' genes and proteins. Results T0901317 inhibited the proliferation of SCL-1 cells in the concentration and time dependant manners. The proportion of SCL-1 cells in G1-phase increased, while that in G2- and S-phase decreased after having been treated with T0901317 (1.0, 10.0 ,20.0 μmol/L) for 24 hours. The result of PCR and Western blot showed that the expression of p21 protein was up-regulated, but the other cell cycle factors had no obviously changes. Conclusion The inhibition of SCL-1 cells proliferation by LXRs agonists is involved in G1-phase arrest, which might be related to the increased expression of p21 factor.
Keywords：Aarcinomasquamous cellSCL-1 cellsLXRsp21G1-phase arrest
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 65-69 )