HPV genotype analysis of cervical secretions from 378 female patients of venereal outpatient

doi:10.11786/sypfbxzz.1674-1293.20170106

Abstract：Objective To explore the human papilloma virus (HPV) genotypes of female cervical secretions in venereal outpatient. Methods Total 378 cases of female patients from STD clinics were recruited. According to the patients with or without high risk exposure and symptoms of condyloma acuminatum, the patients were divided into three groups, namely a group with contact and symptoms (161 cases), a group with contact and without symptoms (117 cases) and a group without contact and symptoms (100 cases). The exfoliated cells were taken and the method of reverse molecular hybridization was adopted to detect the genotype of HPV. Results HPV positive rate of cervical secretions from 378 female cases of venereal outpatient was 56.61% (214/378). Positive rate of the group with contact and symptoms was 98.75% (159/161), and 22 kinds of HPV genotypes were detected, mainly including HPV6 (44.72%), 11(29.19%), 16 (15.53%), 58 (8.69%), 56 (7.45%), 18 (6.83%), 33 (6.83%) and 68 (6.83%). Positive rate of group with contact and without symptoms was 40.17% (47/117), and 14 kinds of HPV genotypes were detected, mainly including HPV6 (17.09%), 11(13.68%), 52 (6.84%), 53 (5.13%), 16 (4.27%), 51(3.42%) and 18 (2.56%). Positive rate of group without contact and symptoms was 8.00% (8/100), and 5 kinds of HPV genotypes were detected, including HPV6 (4.00%), 11(3.00%), 33 (1.00%), 16 (2.00%) and 81(1.00%). Unitary type infection rate and complex infection rate of the group with contact and symptoms and the group with contact and without symptoms had no statistical difference(P>0.05). In the multiple complex infection, three and double infection were the main ones, and the most of them were found to be eight infections. Low-risk type of the group with contact and symptoms was 121cases (76.10%), high-risk pattern was 99 cases (62.26%). Low-risk type of the group with contact and without symptoms was 35 cases (74.46%), high-risk pattern was 26 cases (55.32%). The two groups had high positive rate, but there was no statistical difference(P>0.05). Conclusion HPV6, 58, 56, 11, 16, 18, 33, 68 are primary genotypes of cervical secretions from female cervical in venereal outpatient, which also may be found in healthy examination group. Cervical HPV genetic testing should be strengthened for female venereal patients, and regular follow-up may be carried out on HPV positive patients, especially high risk type and the complex infection patients.