Effect of rutaecarpine ointment on experimental atopic dermatitis in mice and its possible mechanisms
Abstract：Objective To investigate the effect of rutaecarpine ointment on atopic dermatitis in mice and its possible mechanisms. MethodsDNCB was repeatedly applied on the back of NC/Nga mice to establish the animal model of atopic dermatitis. Thirty-six mice were divided into 3 groups: control group, model group and rutaecarpine ointment group, with 12 mice in each group. The treatment of rutaecarpine ointment lasted for 14 days. Levels of several cytokines, including interleukin (IL)-4, IL-5, interferon (IFN)-γ and immunoglobulin E (IgE), from the serum samples were detected by ELISA kit at 8 and 15 days of the experiment. Meanwhile, skin samples underwent the measurement of thickness and weight at 8 and 15 days of the experiment.ResultsRutaecarpine ointment could signiifcantly decrease the skin thickness and weight at 8 and 15 days of the experiment. Meanwhile, rutaecarpine ointment markedly decreased the level of IL-4, IL-5 and IgE, and increased the level of IFN-γ from the serum samples at 8 and 15 days of the experiment.ConclusionThe therapeutic effect of rutaecarpine on atopic dermatitis-like skin lesions may be taken through inhibiting IgE, IL-4 and IL-5 synthesis and promoting the secretion of IFN-γ.
Keywords：DermatitisatopicRutaecarpineCytokinesMice
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 165-167 )