Effects of camptothecin on the expression of CCL20 in HaCaT cells under hypoxia
Abstract：ObjectiveTo investigate the inlfuence of camptothecin (CPT) on the expressions of CCL20 in HaCaT cells under hypoxia, so as to investigate the possible mechanism of CPT in the treatment of plaque psoriasis.MethodsHaCaT cells were cultured respectively in normoxia and hypoxia condition for 12 hours, the CCL20 mRNA expressions were detected by real-time PCR, the secretions of CCL20 protein were detected by ELISA kit. HaCaT cells were divided into ifve groups and treated with different nanomolar (12.5, 25, 50, 100, 200 nmol/L) of CPT, the ifve groups were cultured in hypoxia condition for 12 hours, the secretions of CCL20 protein were detected by ELISA kit.Results There were signiifcantly difference in the expression levels of CCL20 mRNA (given in ΔCT value) between the normoxia group and the hypoxia group (-15.19±0.13 vs-13.70±0.10t=?14.430,P=0.001). The CCL20 protein secretion of HaCaT cells (1×105cells) were increased which cultured in hypoxia for 12 hours, however, there were no significantly difference in the CCL20 protein secretion of HaCaT cells (1×105cells) between the normoxia group and the hypoxia group [(112.18±28.66) pg/ml, (64.36±47.85)pg/ml,t=?1.485, P=0.212]. Hypoxia inducible for 12 hours, CPT of 100, 200 nmol/L signiifcantly decreased the secretion of CCL20 protein (1×105cells) compared with the vehicle control group [(64.35±19.70) pg/ml, (74.35±23.85) pg/ml vs(112.18±28.66)pg/ml,P<0.05].ConclusionThe CCL20 mRNA expression of HaCaT cell is up-regulated in hypoxia, CPT of 100, 200 nmol/L can reduce the secretion of CCL20 protein.
Keywords：PsoriasisCamptothecinCCL20CellhypoxiaHaCaT cells
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 95-97 )