The dynamic changes of interleukin-10 and transforming growth factorβ1 in the mice model of disseminated trichosporonosis
Abstract：Objective To study the dynamic changes of interleukin10(IL-10) and transforming growth factor β1 (TGF-β1) in a mice model of disseminated trichosporonosis, and investigate their possible roles. Methods A total of 50 male BALB/c mice were randomly divided into the experimental group and the control group, and then they were respectively injected with organisms of Trichosporon asahii (T.asahii) or sterile saline via the tail vein. The liver, spleen, kidney, heart, lungs and brain of the mice were aseptically removed and cultured respectively, and then stained with HE and PAS methods;kindey fungal burden were measured;IL-10 and TGF-β1 mRNA levels in spleen were tested with qRT-PCR;the serum level of the IL-10 and TGF-β1 were determined by ELISA and compared with those from normal controls. By using the linear correlation analysis method, the correlation between the quantity of kidney fungal burden and the expression of IL-10 and TGF-β1 were analyzed. Results There were no other pathogenic fungus isolated except T.asahii in experimental group;after the time point of 3 or 7d, mice viscera tissue culture were all positive;14 or 21d, mice kindey tissue culture were all positive;28d, only 2 mice kindey tissue culture were positive. Mice viscera tissue cultures were all negative in control group. Experimental group fungal burdens of kidney at 3, 7, 14, 21 and 28d were (69.5±9.1) cfu/mg, (120.3±11.4) cfu/mg, (36.3±10.9) cfu/mg, (20.8±8.9) cfu/mg and (2.0±2.5) cfu/mg respectively. IL-10 and TGF-β1 mRNA levels of spleen and serum IL-10 and TGF-β1 levels of experimental group increased at 7d, which reached peak at 14d, then decreased to normal levels;at 7, 14 and 21d, comparing with the control group, there were signiifcant difference (P<0.05). Splenic IL-10 mRNA levels in disseminated trichosporonosis correlate positively with serum IL-10 levels(P<0.05), meanwhile splenic TGF-β1 mRNA levels in disseminated trichosporonosis correlate positively with serum TGF-β1 levels (P<0.05) after 7, 14 and 21d of the infection. IL-10 and TGF-β1 mRNA levels of spleen and serum IL-10 and TGF-β1 levels of experimental group correlate positively with fungal burden of kindey(P<0.05), but not after 3 or 28d of the infection (P>0.05). Conclusion IL-10 and TGF-β1 participate in the immune response of disseminated trichosporonosis, they increased at ifrst and then decreased to normal levels, which may reduce the body's ability to remove T.asahii, and provide T.asahii a favorable condition of survival.
Keywords：Disseminated trichosporonosisTrichosporon asahiiIL-10TGF-β1
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 161-166,170 )