Screening of species-speciifc probes forSporothrix schenckiiby PCR-ELISA
SUN Tian
LIU Xiao-ming
ZHANG Zhen-ying
Abstract：ObjectiveTo screen out the highest efficient probe from those that have been reported forSporothrix schenckii (S.schenckii).MethodsFifty strains of culturedS. schenckii and standard strain were used as experimental samples, and 1 strain of Mucor, Aspergillus funigatus, and Monilia albican were used as negative controls. By using the PCR-ELISA format, the most speciifc and sensitive probe was selected from ifve speciifc probes forS. shenckii that have been reported so far, namely probe U26852, probe U26866, probe U26866', probe M85053 and probe AF117945. The sensitivity and speciifcity of the probes were determined based on the color and the optical density.ResultsThe experiment conifrmed that 5 probes were all located on the sequences of the product by sequencing of the PCR products. Under the same hybridization and PCR-ELISA conditions, all the 50 isolates ofS. schenckii had a stronger colouration and higher value of EI to probe U26852 than to the other 4 probes.ConclusionThe probe U26852 (targeting the 28SrRNA gene) forS. schenckii is highly efifcient.
Keywords：Sporothrix schenckiiProbePCR-ELISA
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 403-405,410 )