Application of the lfuorescence quantitative polymerase chain reaction in the diagnosis and typing of genital herpes
Abstract：Objective To estimate the value of the lfuorescence quantitative polymerase chain reaction (FQ-PCR) method in the diagnosis and typing of genital herpes. Methods By employing the FQ-PCR technique, the primers and probes targeted at HSV-1 DNA polymerase gene and HSV-2 glycoprotein D gene fraction were designed and applied to amplify DNA from HSV-1 or HSV-2. Then the PCR reaction system was optimized and evaluated. Swab specimens from suspected patients with genital herpes were detected for HSV by FQ-PCR. Results The FQ-PCR assay showed good speciifcity for detection and typing of HSV, with good linear range (5×102-5×108 copies/ml, r=0.998), and a sensitivity of 5×102 copies/ml, as well as good reproducibility (infra-assay coefifcients of variation was 2.29%and inter-assay coefifcients of variation was 4.76%). Total 186 swab specimens were tested for HSV by FQ-PCR, the positive rate was 23.7%(44/186), among the 44 positive specimens, 8 (18.2%) were positive for HSV-1 with a viral load of 8.5546×106 copies/ml and 36 (81.2%) were positive for HSV-2 with a viral load of 1.9861×106 copies/ml. Conclusion FQ-PCR is a speciifc, sensitive and rapid method for the detection and typing of HSV, which can be used in clinical diagnosis and typing of genital herpes.
Keywords：Genital herpesHerpes simplex virusFluorescent quantitative polymerase chain reactionDiagnosisTyping
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 250-252 )