Effects of retinoic acid on tyrosinase metabolism and melanin content expression in A375 cells
CAO Jun
CHEN Ju-ping
Abstract：ObjectiveTo evaluate the effects of retinoic acid (RA) on melanin content, activity and protein expression of tyrosinase in A375 cells.MethodsCultured A375 cells were classiifed into 4 groups: RA group, 8-methoxypsoralen (8-MOP) group, hydroquinone group, and negative control group received no treatment. Melanin content and tyrosinase activity were determined by NaOH solubilization assay and dopa-oxidation assay respectively at 24, 48 and 72 hours after the addition of RA into the medium. Western blot was performed to detect the protein expression of tyrosinase in A375 cells after 24 hours culture with RA.ResultsThe melanin content and tyrosinase activity decreased in A375 cells after being treated with RA for 24, 48 and 72 hours (P<0.05). The protein (gray scale) in A375 cells at 24 hours expression level of tyrosinase in negative control group was 0.89 ± 0.085, decreased to 0.56 ± 0.044 (t=3.811,P<0.05) after additional treatment with RA.ConclusionRA down-regulates activity of tyrosinase, inhibits protein expression of tyrosinase in A375 cells, as well as the biosynthesis of melanin.
Keywords：MelanocytesTyrosinaseRetinoic acidHydroquinone
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 171-174 )