The inlfuence of murine pathogenicity of Trichosporon asahii after inhibiting lipid raft formation
Abstract：Objective To investigate the relationships between inhibititing lipid raft formation and pathogencity of (Trichosporon asahii, T. asahii) in murine model. Methods Fifty mice were immunosuppressed and divided into 5 groups randomly on the basis of the different suspensions. The experimental groups were inoculated suspensions dealing with amphotericin B which concentrations were 0.2 μg/ml, 0.5μg/ml, 1.0μg/ml, 2.0μg/ml respectively, and the control group was not processed. The death of each group were written down within three weeks, and then the main viscera of the mice were examined by mycologic culture and histopathology. Finally the infection rate were counted. Results The fatality rate and infection rate of countrol group were 80%and 90%respectively. While the number of two groups dealing with amphotericin B which density were 1.0μg/ml, 2.0μg/ml was 37.5%to 20%and 50%to 40%respectively, and was signiifcantly less than the control group(P<0.05). In spite of the number of the other two groups which density were 0.2μg/ml, 0.5μg/ml was decreased, there was no remarkably difference compared with the control group(P>0.05). Histopathology showed acute pyogenous inlfammation and granuloma with arthrospores and mycelia in the tissues. Conclusion Inhibited the lipid raft formation by amphotericin B, the pathogencity of T. asahii decreased apparently. With the increasing of density of amphotericin B, the fatality rate and infection rate of mice decreased gradiently. It was indicated that inhibititing the lipid raft of T. asahii can result in lowering the pathogencity. This discovery provide a clue for a new target of anti-fungal drug.
Keywords：Trichosporon asahiiLipid raftPathogenicityAmphotericin BAnimal experiment
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 92-95 )