Analysis of human papillomavirus genotyping in cervical epithelium in Beijing

doi:10.11786/sypfbxzz.1674-1293.20140201

Abstract：Objective To detect and type human papillomavirus(HPV) by the method of dot blot hybridization reverse-gene chip for investigating their infections in women and their roles in the prevention and treatment in cervical cancer. Methods 1 478 patients from different age were enrolled and the HPV were detected and typed by dot blot hybridization reverse-gene chip. The infection rate of low-risk and high-risk types were compared and the relationship with age were analyzed. Results The total HPV infection rate was 26.05%in 1 478 cervical cell samples, the HPV infection rate of a single type was 19.4%. The predominant type from single infection with HPV was type 16(8.80%) secondly type 58(4.4%). The HPV infection rate of single type, double type and multiple type were 19.4%, 5.1%and 1.6%respectively;the HPV infection rate of high-risk type, low-risk type and mixed type were 17.9%, 4.6%and 3.5%respectively. The positive rate of HPV were 1.2%, 24.0%, 28.8%, 30.0%and 16.0%along with the age group of ≤20, 21 ~ 30, 31, 40, 41 ~ 50 and>50 years respectively, subsequently four age women in the HPV single, double and multiple infection rate difference were statistically signiifcant (χ2=26.475, P<0.01). High-risk and low-risk type infection rate difference were statistically signiifcant (χ2=25.887, P<0.01). Conclusion HPV 16, 58 are the main genotypes of 1 478 women in Beijing district. Gene chip technology had high sensitivity and speciifcity in detecting multiple HPV genotypes in cervical epithelium, which is useful for the prevention and etiology study of cervical cancer.