Isolation and analysis of pathogenic fungi from 206 patients with onychomycosis by multipoint inoculation method
Abstract:Objective To compare the difference between multipoint inoculation and routine method for isolation of pathogenic fungi from nail samples of onychomycosis. Methods Pathogen of onychomycosis was detected by multiple inoculation fungal culture and routine inoculation fungal culture, and the isolated fungi results of both methods were compared. Results The isolation rates of multipoint inoculation method and routine method were 72.73% and 52.96% respectively (P<0.05), and the contamination rates were 8.70%and 4.74%respectively (P<0.01). There were no signiifcant differences between species constituent of dermatophytes (92.00% vs 93.15%), yeasts (6.50% vs 6.85%), moulds (1.50% vs 0%). Both of them were isolated mixed infection, multipoint inoculation method identified 16 and that of routine method was 12. Conclusion Multipoint inoculation method is one way to improve the fungal isolation rate of onychomycosis.
Keywords:OnychomycosisDiagnosisMultipoint inoculationRoutine method
Publication Date:2014-01-01
Online Publishing Date:2025-08-15(First online date of this platform, not the publication date of the document)
Pages:3( 4-6 )
Journal of Practical Dermatology

Journal of Practical Dermatology

ISTIC
ISSN:1674-1293
Year, Vol.(Issue):2014,(1)