Effects of total glucosides of paeony on proliferation of human HaCaT keratinocytes and expression of ICAM-1
Abstract：Objective To evaluate the effects of total glucosides of paeony (TGP) on cell proliferation of human HaCaT keratinocytes and the expression of ICAM-1, and explore the signaling pathways which may be involved in the process. Methods HaCaT cells were stimulated by 500 U/mL IFN-γ, then incubated with TGP of various concentrations (0.5 to 312.5 μg/ml). MTT assay was performed to detect the cell proliferation of HaCaT cells. The expression of ICAM-1 in HaCaT cells was evaluated by enzyme linked immunosorbent assay (ELISA) and lfuorescent immunohistochemical technology. Results The proliferation of HaCaT cells was promoted by TGP of low concentrations (0.5 to 25.0μg/ml), but inhibited by TGP of high concentration (62.5 to 125.0μg/ml). TGP of low concentrations (0.5 to 25.0μg/mL) signiifcantly reduced the expression of ICAM-1. Conclusion TGP signiifcantly inhibited the upregulated ICAM-1 secretion of HaCaT cells which induced by IFN-γ, that may be one of the anti-inlfammatory mechanism of TGP.
Keywords：TGPHaCaT cellsICAM1-1IFN-γ
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 331-334 )