Effects of sirolimus on the autophagy in human skin ifbroblasts
Abstract：Objective To investigate the effects of sirolimus on the autophagy in human skin ifbroblasts(HSFs). Methods HSFs were isolated from the circumcised foreskin of a healthy male, and subjected to primary culture. After 4 to 10 passages, HSFs were incubated with sirolimus of 0, 0.1, 1.0, 10.0 mg/L for 24 h followed by the observation of autophagy. Acndine orange(AO) staining under lforescent microscope and transmission election microscope to detect autophagy, Western blot to detect the expressions of LC3-B and beclin1 in these skin fibroblasts. Results Intracellular acridine orange accumulation increased, and significant differences were observed between the 4 groups, beclin1 and LC3-B expression were up-regulated significantly compared to those untreated skin fibroblasts (all P<0.01). Conclusion The autophagy in HSFs can be upregulated by sirolimus.
Keywords：FibroblastsSirolimusAutophagyLC3-Bbeclin 1
Publication Date：2013-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 259-261 )