The phenotypic regulation mechanism of SRT1720 on hypertrophic scar fibroblasts
YAN Yiwen
ZHANG Ting
ZHENG Zhao
Abstract：Objective To explore the phenotypic regulation mechanism of silence information regulator 1(SIRT1)agonist SRT1720 on hypertrophic scar fibroblasts.Methods From January to June 2022.Department of Burns and Cutaneous Surgery,The First Affiliated Hospital of the Air Force Medical University,according to random number table method,hypertrophic scar fibroblasts(HSF)were divided into PBS control group and SRT1720-treated group.The two groups were respectively treated with equal amounts of PBS and SRT1720 with a final concentration of 2 μmol/L for 24 h.The mRNA expressions of collagen type 1(Collagen Ⅰ)and α-smooth muscle actin(α-SMA)in HSF were detected by a real-time fluorescence quantitative reverse transcription PCR(RT-PCR).The protein ex-pressions of Collagen Ⅰ and α-SMA in HSF were detected by Western blotting.The intracellular expression and localization of Colla-gen Ⅰ and α-SMA of each group in HSF were detected using immunofluorescence method.Statistical analysis was performed using GraphPad Prim 8.0,the data was analyzed by independent sample t test,and statistically significant differences were considered at P＜0.05.Results The mRNA expressions of Collagen Ⅰ and α-SMA in HSF in the SRT1720-treated group was(0.34±0.06)and(0.43±0.08),respectively,which were significantly lower than that in the negative control group of(1.00±0.12)and(1.00±0.13),t=8.57 and 6.79,P＜0.01.The protein expression of Collagen Ⅰ and α-SMA in HSF in the SRT1720-treated group was(0.70±0.05)and(0.64±0.09),which were significantly lower than that in the negative control group of(1.14±0.04)and(1.10±0.05),t=12.61 and 7.37,P＜0.01).Immunofluores-cence staining showed that Collagen Ⅰ and α-SMA were mainly localized in the cytoplasm of HSF,and the expression of Collagen Ⅰ andα-SMA in the SRT1720-treated group was reduced compared with that in the negative control group.Conclusion SRT1720 could sig-nificantly inhibit the expression of Collagen Ⅰ and α-SMA in scar fibroblasts by activating SIRT1,thereby inhibiting extracellular matrix(ECM)synthesis and abnormal transdifferentiation of fibroblasts.
Keywords：Hypertrophic scar fibroblastsCollagen Ⅰα-Smooth muscle actin
Publication Date：2024-07-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 395-398 )