The effect of miR-194-3p on the proliferation of fibroblasts
XU Zhi-shan
CHANG Peng
GUO Bing-yu
HUI Qiang
MA Shu-dan
LI Wei
TAO Kai
Abstract：Objective To analyze the effects of miR-194-3p on the proliferation of fibroblasts in keloids,and provide a theoretical basis for the treatment of keloids.Methods The differential expression of miRNAs with normal tissue was screened by microarray analysis on keloids.Real-time PCR technique was used to verify the expression of miR-194-3p.Western blot and real-time PCR were used to analyze the regulatory effect of miR-194-3p on its target protein RUNX2.After overexpression or inhibition of miR-194-3p in fibroblasts,the proliferation of fibroblasts was observed by MTT experiment.Western blot and real time PCR were used to investigate the regulation of RUNX2 and CDK4,its proliferation-related protein.Results The expression of miR-194-3p was down-regulated in keloids,and it was able to inhibit the expression of RUNX2.MiR-194-3p was able to inhibit the proliferation of fibroblasts by inhibiting CDK4,the proliferation-related protein.Conclusion MiR-194-3p can inhibit the proliferation of keloid fibroblasts.
Keywords：KeloidmiR-194-3pFibroblastsProliferation
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 362-365 )