A study of the influence of recombinant lentivirus LV-hTERT-tumstatin-dNK on the proliferation and apoptosis of melanoma cell A-375
DONG Xiao-shen
ZHENG Xin-yu
Abstract：Objective To construct a recombinant lentivirus LV-hTERT-tumstatin-dNK and explore its efficacy on proliferation and apoptosis of melanoma cells A-375.Methods A-375 and normal human skin cells HFF were infected by different MOI recombinant lentivirus.The cell proliferation was detected by MTT assay and apoptosis was detected by flow cytometry.Results The lentivirus was successfully expressed in A-375,but showed low expression in HFF.The proliferation of A-375 was inhibited by lentivirus,and apoptosis was induced,while there was nearly no change in HFF cells.Conclusion Recombinant lentivirus LV-hTERT-tumstatin-dNK is able to influence the proliferation of melanoma cells A-375 and induce its apoptosis.
Keywords：Recombinant lentivirusMelanomaCell proliferationCell apoptosisDm-dNKTumstatin
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 301-303 )