The inhibitory effect of fangchinoline on hypertrophic scars
SONG Ying-li
WANG Hong-yi
MA Shu-dan
XU Zhi-shan
TAO Kai
Abstract：Objective To explore the inhibitory effect of fangchinoline on hypertrophic scars.Methods After the primary hypertrophic scar fibroblasts were extracted,MTT assay was used to detect the effect of 10 μ mol/L,20 μ mol/L and 40 μ mol/L of fangchinoline on cells.Flow cytometry and Hochest 33258 staining were used to detect the effect of fangchinoline on cell proliferation and apoptosis.The effect of fangchinoline on Cyclin D1 and Bcl-2 in hypertrophic scar fibroblasts was detected by western blot and real time PCR.Results Compared with the DMSO control group,the inhibition rate of 10 μ mol/L,20 μ mol/L and 40 μ mol/L of cells treated with fanchinoline for 24 h were (21.32 ± 4.87)％,(27.13 ± 1.71)％ and (29.86 ± 5.59)％.Fangchinoline was capable of inhibiting the growth of hypertrophic scar fibroblasts and the cell cycle and promote cell apoptosis.Western blot and real time PCR results showed fangchinoline could inhibit the expression of Cyclin D 1 and Bcl-2.Conclusion Fangchinoline can inhibit the growth of hypertrophic scar fibroblasts.
Keywords：Hypertrophic scarFangchinolineProliferationApoptosisInhibit
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 297-300 )