Effect of hyperbaric oxygen preconditioning on superoxide dismutase and malondialdehyde in the random-pattern flap after transplantation

CUI Jia; LIN Bo-jie; PAN Xin-yuan; LI Ya-fan; CAI Jie-yun; PANG Jin-jun; YIN Guo-qian

doi:10.3969/j.issn.1673-7040.2018.04.012

Abstract：Objective To explore the effect of hyperbaric oxygen preconditioning on oxygen-free radicals in random-pattern flaps after transplantation and its relationship to flap survival.Methods A total of 45 SD rats were randomly divided into three groups.An overlength random-pattern flap with a size of 10.0 cm × 2.5 cm was constructed on the back of each SD rat.In the hyperbaric oxygen preconditioning group (n=15),hyperbaric oxygen was given to rats 4 times (2 times a day,for 2 consecutive days) before transplantation.The postoperative hyperbaric oxygen group (n=15) received hyperbaric oxygen 4 times (2 times a day,for 2 consecutive days) after flap transplantation.Rats in the control group (n=15) were in constant pressure atmospheric air both before and after flap transplantation.Tissue samples were taken at 4.0 cm to the pedicle of the flap immediately (day 0) and at days 2,4 and 6 after operation.The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in the flap were detected by the immunohistochemical method,total SOD assay kit,and MDA test kit.Histopathological observation of the flaps was performed at days 2,4,and 6 after operation.Results Immediately after operation (day 0),the content of SOD in the hyperbaric oxygen preconditioning group was significantly higher than that in the postoperative hyperbaric oxygen group (P＜0.01),and the content of MDA was significantly lower than that of the other two groups (P＜0.01).Between the postoperative hyperbaric oxygen group and the control group,the difference in SOD and MDA content was not statistically significant (P＞0.05).On the second day after operation,the content of SOD in the hyperbaric oxygen preconditioning group was significantly higher than that in the postoperative hyperbaric oxygen group and the control group (P＜0.01),and MDA content was significantly lower than that of the other two groups (P＜0.01).On the 4th day postoperative,the SOD content in both the hyperbaric oxygen preconditioning group and the postoperative hyperbaric oxygen group was significantly higher than that in the control group (P＜0.01).However,the difference between the hyperbaric oxygen preconditioning group and the postoperative hyperbaric oxygen group was not statistically significant (P＞0.05).The MDA content of the hyperbaric oxygen preconditioning group was lower than that of the postoperative hyperbaric oxygen group (P＜0.05),and both groups were significantly lower than that of the control group (P＜0.01).On the 6th day after operation,the change trend of SOD content was the same as the 4th day.There was no statistical difference in the MDA content between the hyperbaric oxygen preconditioning group and the postoperative hyperbaric oxygen group (P＞0.05),and both of them were significantly lower than that of the control group (P＜0.01).On the 6th day after operation,the survival rate of the transplanted flap in the hyperbaric oxygen preconditioning group was higher than that in the other two groups (P ＜0.05).Conclusion Hyperbaric oxygen preconditioning can significantly increase the SOD content and can reduce the MDA level in random-pattern flaps after transplantation,and promote the flap survival after transplantation by affecting the balance of oxygen free radicals.