The effect of miR-29a up-regulation on the biological activity of infantile hemangioma endothelial cells
SHEN Di
XU Jian-guo
ZHANG Pei-pei
YANG Chao
DAI Hai-ying
FANG Shuo
XING Xin
Abstract：Objective To explore the effect of miR-29a up-regulation on proliferation,apoptosis and invasion ability of infantile hemangioma endothelial cells in the multiplication period.Methods From 2016 to 2017 the cells were cultured in vitro by the tissue mass adherence method,and endothelial cells derived from the infantile hemangioma were extracted with CD31 magnetic beads.The level of miR-29a in cells was up-regulated through miR-29a overexpression of lentiviral transfection.Cell proliferation was examined by the CCK-8 method.Apoptosis of HemECs was detected by Annexin V-FITC/PI flow cytometry,and the invasion ability was examined by Transwell assay.Results Seventy-two hours after miR-29a overexpression lentiviral transfection,the cell apoptotic ratio was (0.29 ±0.01)％ in the lentiviral transfection group,and (12.54 ± 0.02)％ in the control group.The difference was statistically significant.After upregulation of miR-29a expression in cells,there was no significant influence on the proliferation or invasive ability of the cells.Conclusion Up-regulation of miR-29a has a significant effect on apoptosis,which suggests that miR-29a can modulate biological activity of tumors by changing the apoptosis proportion of endothelial cells.It plays a certain role in the transition from the multiplication period to the regression period of hemangiomas.
Keywords：Infantile hemangiomaVascular endothelial cellApoptosismiR-29a
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 174-176,后插4 )