Regulation of rapamycin on the expression of MMPs and COL-1 in photo-aging fibroblasts
QIN Deng-ke
JIA Chuan-long
LU Yong-zhou
YANG Qing-jian
CHEN Liang
WU Xin-yuan
REN Run-jian
ZHU Jing-jing
GUO Yu
YANG Ping
ZHOU Yi-qun
BI Bo
ZHU Ning-wen
LIU Tian-yi
Abstract：Objective To investigate the influence of rapamycin (RAPA) on the expression of matrix metalloproteinases (MMPs) and the synthesis of type Ⅰ collagen (collagen-Ⅰ) in photo-aging skin fibroblasts (FBs).Methods The best concentration was selected by the effect of RAPA on the proliferative activity of FBs tested by cell counting kit-8 (CCK-8) assay.The photo-aging model of FBs was structured by repeated UVB radiation.The cells were pretreated with RAPA.The influence of RAPA on the expression of MMPs of photo-aging skin FBs was detected by real-time fluorescence quantitative PCR at 24 hours after modeling and the synthesis of collagen Ⅰ was detected by western blotting at 48 h after modeling.Results The proliferative activity of fibroblasts was not affected by RAPA in a low dose manner,5 μm/L was the best concentration for the subsequent treatment.The UVB irradiation increased the expressions of MMP-1,2,9 of FBs.RAPA pretreatment significantly decreased expression of MMPs caused by UVB and increased the expression of collagen Ⅰ.Conclusion RAPA can maintain the metabolic balance of dermal ECM by decreasing the expression of MMPs and increasing the synthesis of collagen Ⅰ in photo-aging FBs,and to a certain extent,alleviate the degree of cell photoaging.
Keywords：FibroblastRapamycinPhotoagingMatrix metalloproteinasesCollagen-Ⅰ
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 723-725,732 )