Study of the regenerative mechanism of adipose-derived stem cells on SD rats with sciatic nerve defects
SONG Qi-bin
WANG Zhe
WANG Yu-ling
LI Yang
LIN Shuang
Abstract：Objective To explore the efficacy of the application of poly lactic co glycolic acid (PLGA) nerve tube loaded with adipose-derived stem cells in the repair of sciatic nerve defects in SD rats.Methods The adipose-derived stem cells were extracted from 10 SD rats as an experimental group.Another 10 SD rats were selected as the control group.A 5.0 mm defect model of the unilateral sciatic nerve of SD rats was made.Twelve weeks after operation,the effect of nerve regeneration was evaluated through electroneurophysiological examination,histological observation,and gastrocnemius wet weight.Results After 12 weeks,7 rats from the experimental group and 8 rats from the control group survived.The sciatic nerve conduction rate was (12.39 ± 0.71) ms in the experimental group,and (8.13 ± 0.87) ms in the control group.The wet weight ratio of the gastrocnemius muscle was (47.69 ± 0.24) in the experimental group and (35.77 ± 0.73) in the control group.All differences were statistically significant.The number of myelinated nerve fibers were less and scattered in the control group.A larger number of myelinated nerve fibers and nerve fascicles formed in the experimental group.Conclusion ADSCs can promote the growth of the peripheral nerve in SD rats.
Keywords：Adipose-derived stem cellsSD ratsPeripheral nerve defect modelPLGA nerve tube
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 720-722 )