Experimental research of reprogramming of adipose-derived stem cells using human mammary epithelial cell extracts
YANG Yan-qing
JIANG Chang-yan
ZHANG Jie
XU Qi-jun
WANG Hai-Yan
Abstract：Objective To explore the feasibility of human mammary epithelial cell extracts in the reprogramming and conversion of human adipose-derived stem cells (hADSCs) to epithelial cells.Methods The ADSCs were isolated by the collagenase digestion method and cultured.The human mammary epithelial cells were obtained by ultrasonic emulsification.The extracts were acquired through high speed centrifugation and frozen for further use.The third generation of hADSCs were cultured and treated with streptolysin O (SLO).The mammary epithelial cell extracts were added into the 6 cases in the experimental group,but not added into the 6 cases in the control group.The morphological changes of ADSCs were observed during the cell culture process.The changes in zona occludens protein 1 (ZO1) and cytokeratin 18 (CK18) expression in each group were detected by Western blot.Results Flow cytometry showed that the expression of CD13,CD29,CD44,CD90,and CD105 were positive and the expression of CD45 was negative.At 16 days after ADSCs reprogramming in the experimental group,the cell morphology began to turn into an epithelial-like circle shape.Western blot results demonstrated that the expression of ZO1 and CK18 in the experimental group increased more significantly than that in the control group at the 18th day (P＜0.05).Conclusion Human mammary epithelial cell extracts can reprogram ADSCs and cause them to transform into epithelial cells.
Keywords：Mammary epithelial cellAdipose-derived stem cellsReprogrammingCell extracts
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 709-711 )