Effects of different diameter injection needles on the activity of adipocytes

AN Ran; SUN Jia-ming; WANG Jie-cong

doi:10.3969/j.issn.1673-7040.2017.11.009

Abstract：Objective To detect the activity of adipocytes of injection needles of different diameters and to study the effects of injection needles with different diameters on the activity of adipocytes.Methods Two hundred milliliters of fat granules obtained by traditional negative pressure suction were prepared.After filtration and rinse with double layer gauze,the fat granules were then transferred into 20 syringes (5 ml) and randomly divided into 4 groups (n=5).One was the control group.In the other three groups,different diameter injection needles (12 G、16 G、and 18 G) were employed using 5 ml syringes to separate the fat granules,then fat granules were transferred into centrifuge tubes (15 ml) at a constant rate.Adipocyte activity was detected in the control group and the other three groups with Calcein-AM/Hoechst 33342 fluorescence staining.The rate of viable cells was calculated and the results of the use of different diameters of injection needles on the activity of adipocytes were compared.Results The injection needle (12 G、16 G、18 G) process was smooth without obvious resistance.The viability of fat particles injected in the control group and the groups with the three different diameter injection needles (12 G、16 G、18 G) detected by Calcein-AM/Hoechst 33342 fluorescence stain was (83.53 ± 7.92)％,(82.62 ± 8.09)％,(75.78 ± 10.94)％,and (68.45 ± 11.24)％,the difference among the three groups (12 G、16 G、18 G) was statistically significant (P＜0.05),but was not statistically significant between the control group and the 12 G group (P＞0.05).Conclusion Different diameters of injection needles have differing impacts on the activity of fat adipocyte.By decreasing the diameter of the injection syringe,live cells are reduced in fat particles when a certain amount of fat particles per unit of time is injected.Increasing the diameter of the needle can increase the ratio of live cells after injection.