RNA-seq study of the hair follicular bulge region in androgenetic alopecia
QU Qian
MIAO Yong
LIU Xiao-min
SHI Pan-li
FAN Zhe-xiang
GUO Zhen-he
HU Zhi-qi
Abstract：Objective To explore the differencein hair follicle bulge in the frontal andoccipital areas between people with normal hair growth and patients with androgenetic alopecia (AGA).The pathological mechanism of the hair follicular bulge region in AGA patients will be investigated.Methods Hair follicles were harvested from the frontal andoccipital areas of 10 AGA patients and 10 healthy volunteers.The hair follicular bulge was obtained through micro-dissection for further RNA-seq study.The transcription expression difference of the above tissues was detected and analyzed with second generation high-throughput sequencing technology.The key mechanisms of change in the hair follicular bulge in AGA was further exploredby function annotation and bioinformatics analysis.Results There were 214 differentially-expressed genes (DEGs) between the frontal and occipital area in the AGA patients.There were 511 and 399 DEGs in the frontal and occipital areas between the AGA patients and the healthy volunteers.The functional analysis of differential genes was mainly concentrated ontissue remodeling and DNA damage.Conclusion In the development of AGA,important pathological changes occur in the hair bulge region,including tissue remodeling and higher DNA damage.
Keywords：Androgenetic alopeciaStem cellHair folliclebulgeBulge regionDNA damage
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 519-522 )