Immunohistochemical staining analysis of contractile protein after botulinum toxin type A injection in human masseter muscle
WANG Jun
ZHU Shun-wen
MA Hao
XIE Zhi-wei
HU Ya-nuan
MA Fu-shun
Abstract：Objective To observe the change of actin and myosin after botulinum toxin type A injection in human masseter muscle.Methods A total of 60 specimens were collected from patients with benign hypertrophic masseter muscle.Thirty specimens without botulinum toxin type A injection were selected as the control group,and the other 30 specimens with botulinum toxin type A injection as the experimental group.Immunohistochemical staining was applied for all specimens in both groups.The expression of actin and myosin was observed under a light microscope.The optical density value was measured and statistically analyzed.Results The expression of actin and myosin in the experimental group was decreased.The actin,in particular,decreased significantly.Compared with the control group,the difference was statistically significant (P＜0.05).The expression of actin and myosin reached their lowest level at 12 weeks after Botulinum toxin type A injection,then recovered gradually.The expression of actin and myosin recovered to near the control group level at 24 weeks,and the difference in optical density values between the experimental group and the control group was not statistically significant (P＞0.05).Conclusion Botulinum toxin type A may have an effect on the expression of contractile protein and lead to lower bite force.
Keywords：Botulinum toxin type AMasseter muscleMyosinActin
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 468-471 )