The expression and significance of Shh and Gli-1 in human hypertrophic scar and normal skin fibroblasts in vitro
ZHAO Xiao-kai
LOU Ji-he
XIA Cheng-de
TIAN She-min
NIU Xi-hua
LI Shu-ren
ZHANG Jian
PAN Shu
Abstract：Objective To investigate the expression of Shh and Gli-1 in the Sonic hedgehog signaling pathway in human hypertrophic scar and normal skin fibroblasts in vitro,and to explore the significance of this signaling pathway in hypertrophic scar formation.Methods The expression of Shh and Gli-1 in 15 samples of human hypertrophic scar and normal skin fibroblasts was detected by western blot and immunocytochemistry.Results (1) Western blot showed that both normal human skin and hypertrophic scar fibroblasts had Shh and Gli-1 protein expression,and their expressions in the hypertrophic scar fibroblasts [(0.640 ± 0.093) and (0.560 ± 0.120)] were higher than that in the normal human skin fibroblasts [(0.330 ± 0.023) and (0.340 ± 0.050)].The difference was statistically significant (P ＜ 0.05).(2) Immunocytochemistry showed that both Shh and Gli-1 were expressed in both hypertrophic scar and normal skin fibroblasts.The level of Shh and Gli-1 protein was significantly raised.Conclusion The expression of Shh and Gli-1was elevated in hypertrophic scar fibroblasts which indicated that the Sonic hedgehog signaling pathway may play an important role in the occurrence of hypertrophic scars.
Keywords：Hypertrophic scarHedgehog signaling pathwayShhGli-1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 335-337,357 )