The effect of botulinum toxin type A on the proliferation of human keloid fibroblasts
YANG Yan-qing
ZHANG Jie
XU Qi-jun
WU Xi-xi
HUANG Yan
JIANG Chang-yan
Abstract：Objective To investigate the effect of botulinum toxin type A (BTXA) on the proliferation of human keloid fibroblasts.Methods Human keloid fibroblasts were isolated and cultured in vitro,and a medium containing BTXA (diluted concentration of 50 U/ml) was used to interfere with cell growth.The experimental group was divided into 2 gToups of 10 cases each.Different amounts of BTXA were added to the cell medium of each group (1.0 μ l and 2.5 μ l),including 1 × 106 cells.The effect of BTXA on each group was compared.The blank control group (cell medium without BTXA) included 8 cases.At 72 hours after co-culture,immunohistochemistry (LsAB method) was adopted to detect the expression of Ki67.Results The rate of cell proliferation was slow and the number of cells decreased in the experimental group.The positive expression rate of Ki67 was 63.2％ in the blank control group and 33.6％ in the experimental group,showing a significant difference between the two groups (P＜0.01).The positive expression rate of Ki67 was 33.6％ in the experimental group with the BTXA dose of 1.0 μl and 25.6％ in the experimental group with the BTXA dose of 2.5 μl,showing a significant difference between the two groups (P＜0.05).Conclusion The proliferation of human keloid fibroblasts can be inhibited by BTXA,and the larger the dosage,the more obvious the inhibition effect.
Keywords：KeloidFibroblastBotulinum toxin type ACell proliferation
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 332-334 )