Study of KU-0063794 on the inhibition of proliferation and migration of hypertrophic scar fibroblasts
YU Zhou
SONG Ya-juan
SU Ying-jun
CHEN Lin
MA Xian-jie
PENG Pai
Abstract：Objective To explore the effect of KU-0063794 on the proliferation and migration of hypertrophic scar fibroblasts iso lated from human hypertrophic scar tissue.Methods Hypertrophic scar fibroblasts were isolated and cultured in vitro,and their morphological features were analyzed.The hypertrophic scar fibroblast cells were cultured in fetal bovine serum-free high-glucose Dulbecco Modified Eagle Medium for 12 to 16 hours,then divided into two groups:the control group (treated with DMSO) and KU-0063794 treated groups [treated with different doses of KU-0063794 (1.0,2.5,5.0,10.0,20.0 μm)] for 24 h.The Proliferation activity of hypertrophic scar fibroblasts was detected with an MTS kit.The migration ability of the hypertrophic scar fibroblasts was analyzed by wound healing assay.Results Isolated hypertrophic scar fibroblasts had good morphology.The proliferation and migration of hypertrophic scar fibroblasts could be inhibited by KU-0063794 in a concentration-dependent manner,and the difference was statistically significant (P＜0.05).Conclusion Hypertrophic scar fibroblasts were successfully isolated.KU-0063794 had the ability to inhibit hypertrophic scar fibroblast proliferation and migration.
Keywords：Hypertrophic scarFibroblastKU-0063794ProliferationMigration
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 328-331 )