Establishment of a simultaneous transplantation mouse model of allogeneic heterotopic heart and skin flap
LIU Shi-qiang
DING Jian-ke
ZHANG Dong-liang
CHEN Chen
CHEN Jian-wu
ZHUANG Ran
SU Ying-jun
GUO Shu-zhong
Abstract：Objective We aimed to develop a model of concomitant heart and composite tissue transplant in mice,which could be used to compare the possible surgical results.Methods Isograft transplants were performed among C57BL/6 mice.The donor's heart was harvested with the right common carotid artery,then the aorta and pulmonary artery were anastomosed to the recipient's right common carotid artery and external jugular vein,and the donor's abdominal flap vessels were connected to the donor's right common carotid artery and recipient's left external jugular vein,respectively.Heart viability was assessed by daily palpation,and the skin flap was observed every day.Finally,the grafts were assessed histologically.Results Indefinite allograft survival (＞100 days) was achieved in the isograft transplant group.The mean total operative time was (193.6 ± 7.8) minutes with the average warm ischemia time of heart and skin flap was (14.4 ± 1.7) minutes and (28.0 ± 4.2) minutes,respectively.Histologic examination displayed viable grafts without necrosis or fibrosis.Conclusion We succeeded in developing a technically feasible and reliable model of simultaneous heart and composite tissue transplant in mice which may be used in the research of mutual immunologic influence and split rejection between composite tissue and solid organ.
Keywords：Composite tissue allotransplantationSolid organ transplantationCuff techniqueVessel bridgeMice model
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 166-169 )