Analysis of expression of the CD26 marker on keloid fibroblasts
XIN Yu
Melia Bogari
Zin Mar Aung
CHEN Wei
CHAI Gang
ZHANG Yan
Abstract：Objective To analyze the expression of the CD26 marker on normal skin fibroblasts (NFs) and keloid fibroblasts (KFs). Methods Normal skin tissues in the NFs group were obtained from individuals who underwent abdominal plastic resection. Keloid tissues in the breast and abdomen were harvested in surgery from patients who did not receive any treatment for their keloids. Then NFs and KFs were obtained through the enzyme digestion method. Flow cytometry technology was used to analyze the expres-sion of the CD26 marker on NFs and KFs, and isolate the CD26 positive expressed (CD26+) and CD26 negative expressed (CD26-) cell population of NFs and KFs. Then the cell morphology of CD26-/+ fibroblasts were observed after 4 days in vitro culture by in-verted microscope. Results The CD26 marker was expressed in both KFs and NFs. KFs exhibited a higher expression rate of CD26 (46.65±13.97)% than NFs with that of (24.75±16.85)% (P<0.01). Observation of cell morphology after 4 days in vitro culture ex-hibited similar shape and cell density between CD26- cell population and CD26+ cell population in both the NFs and KFs groups. Conclusion KFs exhibited a higher expression rate of CD26 than NFs, while the CD26- and CD26+cell population in both the NFs and KFs groups showed similar morphological characteristics.
Keywords：CD26 markerFibroblastsKeloidNormal skinFlow cytometry technology
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 39-42 )